The Chromium Single Cell ATAC Solution produces Illumina® sequencer-ready libraries.
Single Cell ATAC Libraries (v1, v1.1, and v2 Chemistries)
Important
Sequencing requirements of Multiome ATAC libraries require different considerations, refer to the Sequencing Requirements for Single Cell Multiome ATAC + Gene Expression page for specifications.
Supported Sequencers:*
- Illumina® NovaSeq 6000
- Illumina® HiSeq 3000/4000
- Illumina® HiSeq 2500
- Illumina® NextSeq 500/550
- Illumina® NextSeq 1000/2000
- Illumina® MiSeq
Recommended Sequencing Depth: 25,000 read pairs per nucleus (50,000 individual reads. 25,000 from R1, 25,000 from R2)
Dual-Indexed Sequencing Run: Single Cell ATAC libraries are dual-indexed.
PhiX Spike-In Recommendations: 1%.
| Read | Read 1 | i7 Index | i5 Index | Read 2 |
|---|---|---|---|---|
| Purpose | Transposed DNA | Sample Index | 10x Barcode | Transposed DNA |
| Length | 50 | 8 | 16 | 50 ** |
* Do not pool Chromium Single Cell ATAC libraries with other 10x libraries while sequencing
** Can be 1 bp shorter to accommodate NovaSeq v1.0 100 cycle kits