The Single Cell Gene Expression Solution coupled with Feature Barcoding technology enables profiling of gene expression in conjunction with additional cellular features from the same single cell. Specifically, the Single Cell Gene Expression and CRISPR Screening Solution provides a high-throughput and scalable approach to obtain gene expression profiles along with CRISPR-mediated perturbation phenotypes in transduced cells via direct capture of poly-adenylated mRNAs and guide RNAs (sgRNAs). This document provides specifications for engineering Feature Barcoding technology compatible sgRNAs for use with:

• Chromium Single Cell 3ʹ Reagent Kits v3 with Feature Barcoding technology for CRISPR Screening (CG000184)
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for CRISPR Screening (CG000205)
• Chromium Next GEM Single Cell 3’Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for CRISPR Screening (CG000316)
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for CRISPR Screening and Cell Multiplexing (CG000389)
• Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1with Feature Barcode technology for CRISPR Screening (CG000418)
• Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 with Feature Barcode technology for CRISPR Screening and Cell Multiplexing (CG000421)