Support homeSingle Cell Gene ExpressionDocumentationLibrary Construction
Workflow Update for Chromium Next GEM Assays

Workflow Update for Chromium Next GEM Assays

Learn more about a change in the recommended loading technique

What do I need to know?

  • We are updating our best practice loading guidance for 50% glycerol solution from 45 µl to 150 µl in unused recovery wells for all Next GEM–based assays.
  • This change improves consistency, ensuring that the recovery well loading volume is large enough to guard against deviations from our best practice recommendations.
  • Additional best practices for loading chips should still be followed: e.g., loading at the center of the well near the bottom and raising the pipette tip as liquid is dispensed.

Please note this change is immediate and does not alter the loading recommendations for HT workflows or GEM-X workflows.

Chromium Next GEM User Guides impacted by this change

AssayDocument NumberUser Guide Name
Chromium Next GEM Training KitCG000210Chromium Next GEM Training Kit User Guide
Chromium Next GEM Single Cell Gene Expression 3’ v3.1CG000315Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index)
Chromium Next GEM Single Cell Gene Expression 3’ v3.1CG000316Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index) with Feature Barcoding technology for CRISPR Screening
Chromium Next GEM Single Cell Gene Expression 3’ v3.1CG000317Chromium Single Cell 3' Reagent Kits User Guide (v3.1- Dual Index) with Feature Barcoding technology for Cell Surface Protein
Chromium Next GEM Single Cell Gene Expression 3’ v3.1CG000388Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index) with Feature Barcoding technology for Cell Multiplexing
Chromium Next GEM Single Cell Gene Expression 3’ v3.1CG000389Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index) with Feature Barcoding technology for CRISPR Screening and Cell Multiplexing
Chromium Next GEM Single Cell Gene Expression 3’ v3.1CG000390Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index) with Feature Barcoding technology for Cell Surface Protein and Cell Multiplexing
Chromium Next GEM Single Cell Immune Profiling 5’ v2CG000330Chromium Single Cell 5' Reagent Kits User Guide (v2 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein and Immune Receptor Mapping
Chromium Next GEM Single Cell Immune Profiling 5’ v2CG000331Chromium Single Cell 5' Reagent Kits User Guide (v2 Chemistry Dual Index)
Chromium Next GEM Single Cell Immune Profiling 5’ v2CG000510Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) with Feature Barcode technology for CRISPR Screening
Chromium Next GEM Single Cell Immune Profiling 5’ v2CG000511Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) with Feature Barcode technology for CRISPR Screening and Cell Surface Protein
Chromium Next GEM Single Cell Immune Profiling 5’ v2CG000591*Chromium Next GEM Single Cell 5’ Reagent Kits v2 (Dual Index) with Feature Barcode Technology for Barcode Enabled Antigen Mapping (BEAM)
Chromium Next GEM Single Cell Immune Profiling 5’ v2CG000592*Chromium Next GEM Single Cell 5’ Reagent Kits v2 (Dual Index) with Feature Barcode Technology for Barcode Enabled Antigen Mapping (BEAM) & Cell Surface Protein
Chromium Next GEM Single Cell Gene Expression FlexCG000477Chromium Fixed RNA Profiling Reagent Kits for Singleplexed Samples With Feature Barcode technology for Protein
Chromium Next GEM Single Cell Gene Expression FlexCG000527Chromium Fixed RNA Profiling for Multiplexed Samples
Chromium Next GEM Single Cell Gene Expression FlexCG000673Chromium Fixed RNA Profiling Reagent Kits for Multiplexed Samples with Feature Barcode technology for Protein using Barcode Oligo Capture
Chromium Next GEM Single Cell Gene Expression FlexCG000674Chromium Fixed RNA Profiling Reagent Kits for Singleplexed Samples with Feature Barcode technology for Protein using Barcode Oligo Capture
Chromium Next GEM Single Cell Gene Expression FlexCG000691Chromium Fixed RNA Profiling Reagent Kits for Singleplexed Samples
Chromium Single Cell ATACCG000496*Chromium Single Cell ATAC Reagent Kits User Guide (v2 Chemistry)
Chromium Single Cell Multiome ATAC + Gene ExpressionCG000338*Chromium Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide

*User Guide updates for CG000591, CG000592, CG000496, CG000338 are in progress and will be uploaded to the 10x Genomics Support site once available. In the interim, the updated glycerol loading guidance can be followed from CG000315 step 1.2.a.

For additional information, please reach out to support@10xgenomics.com.

Document Type
Product Notification
Last Modified
July 29, 2024