Multiomic characterization of the tumor microenvironment in FFPE tissue by simultaneous protein and gene expression profiling.
The tumor microenvironment (TME) is a highly heterogeneous structure; by knowing and understanding its composition researchers can gain critical insights in disease progression and therapeutic response. Utilizing the 10x Genomics Visium Spatial Gene and Protein Expression for FFPE we demonstrate a streamlined multiomic spatial assay that integrates histopathological evaluation with transcriptome-wide gene expression and multiplexed protein expression profiling from the same FFPE tissue section. By implementing whole transcriptome probes and an immunology antibody panel, we applied this method to measure multiomic expression within the TME of human breast and other tumor samples using whole transcriptome probes and an immunology antibody panel. Together, this spatial and multiomic approach provides an unbiased and comprehensive understanding of cellular behavior in and around tumors.
The tumor microenvironment (TME) is a highly heterogeneous structure; by knowing and understanding its composition researchers can gain critical insights in disease progression and therapeutic response. Utilizing the 10x Genomics Visium Spatial Gene and Protein Expression for FFPE we demonstrate a streamlined multiomic spatial assay that integrates histopathological evaluation with transcriptome-wide gene expression and multiplexed protein expression profiling from the same FFPE tissue section. By implementing whole transcriptome probes and an immunology antibody panel, we applied this method to measure multiomic expression within the TME of human breast and other tumor samples using whole transcriptome probes and an immunology antibody panel. Together, this spatial and multiomic approach provides an unbiased and comprehensive understanding of cellular behavior in and around tumors.