Improving Single Cell Characterization with Simultaneous Gene Expression and Cell Surface Protein Measurements at Scale
Cells establish their function, identity, and state through the careful orchestration of complex molecular mechanisms leading to gene expression. While gene expression can be measured by type and quantity of mRNA transcripts produced, it is well known that the abundance and isoforms of expressed proteins cannot always be inferred directly from mRNA readout alone. Thus, to more accurately characterize cellular identity, state, and function, it is important to evaluate gene expression at both transcript and protein levels.
Cells establish their function, identity, and state through the careful orchestration of complex molecular mechanisms leading to gene expression. While gene expression can be measured by type and quantity of mRNA transcripts produced, it is well known that the abundance and isoforms of expressed proteins cannot always be inferred directly from mRNA readout alone. Thus, to more accurately characterize cellular identity, state, and function, it is important to evaluate gene expression at both transcript and protein levels.